High Resolution Chromatography: A Practical Approach(Practical Approach Series).Product Details
Hardcover: 336 pages Publisher: Oxford University Press, USA; 1 edition(July 10, 1999) Language: English ISBN-10: 0199636494 ISBN-13: 978-0199636495 Product Dimensions: 9.3 x 6.2 x 0.9 inches Shipping Weight: 1 ounces.
Hardcover: 336 pages Publisher: Oxford University Press, USA; 1 edition(July 10, 1999) Language: English ISBN-10: 0199636494 ISBN-13: 978-0199636495 Product Dimensions: 9.3 x 6.2 x 0.9 inches Shipping Weight: 1 ounces.
Product DescriptionIsolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques,detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions usingpeptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.
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