The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.
Contents
Part I Introduction
1 An Overview of HPTLC: A Modern Analytical Technique with
Excellent Potential for Automation, Optimization, Hyphenation,
and Multidimensional Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
MM. Srivastava
Part II Fundamentals, Principle and Advantages of HPTLC
2 Fundamentals and Theory of HPTLC-Based Separation . . . . . . . . . . . . . . 27
Prasad S. Variyar, Suchandra Chatterjee, and Arun Sharma
3 Experimental Aspects and Implementation of HPTLC . . . . . . . . . . . . . . . . 41
Rashmin B. Patel, Mrunali R. Patel, and Bharat G. Batel
4 High-Performance Thin-Layer Chromatography: Excellent
Automation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Dilip Charegaonkar
Part III Applications of HPTLC Separation
5 Multidimensional and Multimodal Separations by HPTLC
in Phytochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Lukasz Ciesla and Monika Waksmundzka-Hajnos
6 Stability-Indicating HPTLC Determination of Imatinib Mesylate
in Bulk Drug and Pharmaceutical Dosage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
P. Musmade, N. Vadera, and G. Subramanian
7 HPTLC Fingerprint Analysis: A Quality Control
for Authentication of Herbal Phytochemicals . . . . . . . . . . . . . . . . . . . . . . . . . 105
Mauji Ram, M.Z. Abdin, M.A. Khan, and Prabhakar Jha
xi
8 HPTLC in Herbal Drug Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Machindra J. Chavan, Pravin S. Wakte, and Devanand B. Shinde
9 HPTLC Determination of Artemisinin and Its Derivatives
in Bulk and Pharmaceutical Dosage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
Suraj P. Agarwal and Shipra Ahuja
10 TLC/HPTLC in Biomedical Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
A. Mohammad and A. Moheman
11 Analytical Aspects of High Performance Thin Layer
Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Gunawan Indrayanto
12 Quantitative Analysis and Validation of Method
Using HPTLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
Pinakin Dhandhukia and Janki N. Thakker
13 Quantification of Low Molecular Mass Compounds
Using Thermostated Planar Chromatography . . . . . . . . . . . . . . . . . . . . . . . 223
Paweł K. Zarzycki
Part IV HPTLC and its Future to Combinatorial Approach
14 Basic Principles of Planar Chromatography and Its Potential
for Hyphenated Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
Tomasz Tuzimski
15 HPTLC–MS Coupling: New Dimension of HPTLC . . . . . . . . . . . . . . . . . 311
Ajai Prakash Gupta and Suphla Gupta
16 TLC/HPTLC with Direct Mass Spectrometric Detection:
A Review of the Progress Achieved in the Last 5 Years . . . . . . . . . . . . . 335
Jurgen Schiller, Beate Fuchs, Kristin Teuber, Ariane Nimptsch,
Kathrin Nimptsch, and Rosmarie Su¨ß
17 Scanning Diode Laser Desorption Thin-Layer Chromatography
Coupled with Atmospheric Pressure Chemical Ionization Mass
Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 365
Song Peng, Norman Ahlmann, Michael Edler, and Joachim Franzke
18 HPTLC Hyphenated with FTIR: Principles, Instrumentation
and Qualitative Analysis and Quantitation . . . . . . . . . . . . . . . . . . . . . . . . . . . 385
Claudia Cimpoiu
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395
xii Contents
List of Contributors
Abdul Moheman Department of Applied Chemistry, Faculty of Engineering and
Technology, Aligarh Muslim University, Aligarh 202002, India
Ajai Prakash Gupta Public Health Engineering Department, IIIM-CSIR, Jammu&
Kashmir, India
Ali Mohammad Department of Applied Chemistry, Faculty of Engineering and
Technology, Aligarh Muslim University, Aligarh 202002, India
Ariane Nimptsch University of Leipzig, Medical Department, Institute of Medical
Physics and Biophysics, Ha¨rtelstr, 16/18, D-04107 Leipzig, Germany
Arun Sharma Food Technology Division, Bhabha Atomic Research Centre,
Mumbai 400085, India
Beate Fuchs University of Leipzig, Medical Department, Institute of Medical
Physics and Biophysics, Ha¨rtelstr, 16/18, D-04107 Leipzig, Germany
Bharat G. Patel A. R. College of Pharmacy and G. H. Patel Institute of Pharmacy,
Sardar Patel University, University of Leipzig Gujarat, Vallabh Vidyanagar 388
120, India
Claudia Cimpoiu Faculty of Chemistry and Chemical Engineering, Babes Bolyari
University, Cluj Napoca, Romania
Devanand B. Shinde Department of Chemical Technology, Dr. Babasaheb
Ambedkar Marathwada University, Aurangabad 431001, India
G. Subramanian Department of Pharmaceutical Quality Assurance, Manipal College
of Pharmaceutical Sciences, Manipal, Karnataka 576104, India
Gunawan Indrayanto Plant Biotechnology Research Group and Assessment Service
Unit Faculty of Pharmacy, Airlangga University, Surabaya 60286, Indonesia
Janki N. Thakker Department of Biotechnology, PD Patel Institute of Applied
Science, Charutar University of Science & Technology, Education Campus
Changa, 388421 Gujarat, India
xiii
Joachim Franzke ISAS—Institute for Analytical Sciences, Bunsen-Kirchhoff-
Straße 11, 44139, Otto-Hahn-Straße 6b, 44227 Dortmund, Germany
Jurgen Schiller University of Leipzig, Medical Department, Institute of Medical
Physics and Biophysics, Ha¨rtelstr, 16/18, D-04107 Leipzig, Germany
Kathrin Nimptsch University of Leipzig, Medical Department, Institute of Medical
Physics and Biophysics, Ha¨rtelstr, 16/18, D-04107 Leipzig, Germany
Kristin Teuber University of Leipzig, Medical Department, Institute of Medical
Physics and Biophysics, Ha¨rtelstr, 16/18, D-04107 Leipzig, Germany
Lukasz Ciesla Department of Inorganic Chemistry, Faculty of Pharmacy, Medical
University of Lublin, Lublin, Poland
M.A. Khan Centre for Transgenic Plant Development, Department of Biotechnology,
Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110062,
India
M.Z. Abdin Centre for Transgenic Plant Development, Department of Biotechnology,
Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110062,
India
Machindra J. Chavan Department of Pharmacognosy, Amrutvahini College of
Pharmacy, Sangamner, S.K. Dist-Ahmednagar (M.S) 422 605, India
ManMohan Srivastava Department of Chemistry, Faculty of Science, Dayalbagh
Educational Institute, Dayalbagh, Agra 282110, India
Mauji Ram Centre for Transgenic Plant Development, Department of Biotechnology,
Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110062,
India
Michael Edler ISAS—Institute for Analytical Sciences, Bunsen-Kirchhoff-Straße
11, 44139 Otto-Hahn-Straße 6b, 44227 Dortmund, Germany
Monika Waksmundzka Hajnos Department of Inorganic Chemistry, Faculty of
Pharmacy, Medical University of Lublin, Lublin, Poland
Mrunali R. Patel Indukaka Ipcowala College of Pharmacy, Sardar Patel University,
New Vallabh Vidyanagar, 388 121 Gujarat, India
N. Vadera Department of Pharmaceutical Quality Assurance, Manipal College of
Pharmaceutical Sciences, Manipal, Karnataka 576104, India
Norman Ahlmann ISAS—Institute for Analytical Sciences, Bunsen-Kirchhoff-
Straße 11, 44139 Otto-Hahn-Straße 6b, 44227 Dortmund, Germany
P. Musmade Department of Pharmaceutical Quality Assurance, Manipal College
of Pharmaceutical Sciences, Manipal, Karnataka 576104, India
xiv List of Contributors
Paweł K. Zarzycki Section of Toxicology and Bioanalytics, Koszalin University
of Technology, S ´ niadeckich 2, 75-453 Koszalin, Poland
Pinakin Dhandhukia Ashok and Rita Patel Institute of Integrated Study &
Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar, 388
121 Gujarat, India
Prabhakar Jha Department of Botany, Faculty of Science, Jamia Hamdard,
Hamdard Nagar, New Delhi 110062, India
Prasad S. Variyar Food Technology Division, Bhabha Atomic Research Centre,
Mumbai 400085, India
Pravin S. Wakte Department of Chemical Technology, Dr. Babasaheb Ambedkar
Marathwada University, Aurangabad 431 001 (M.S), India
Rashmin B. Patel A. R. College of Pharmacy and G. H. Patel Institute of Pharmacy,
Sardar Patel University, Vallabh Vidyanagar, 388 120 Gujarat, India
Rosmarie Su¨ß University of Leipzig, Medical Department, Institute of Medical
Physics and Biophysics, Ha¨rtelstr, 16/18, D-04107 Leipzig, Germany
Shipra Ahuja Department of Pharmaceutics, Jamia Hamdard University, Hamdard
Nagar, New Delhi 110062, India
Song Peng ISAS—Institute for Analytical Sciences, Bunsen-Kirchhoff-Straße 11,
44139 Otto-Hahn-Straße 6b, 44227 Dortmund, Germany
Tomasz Tuzimski Department of Physical Chemistry, Faculty of Pharmacy,
Medical University of Lublin, Lublin, Poland.
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