High Performance Thin-layer Chromatography (Journal of chromatography library). By
Albert Zlatkis, R.E. KaiserPreface and Introduction
The publication of this book appears at a time when many colleagues who are
actively involved in analytical research, consider thin-layer chromatography as one
of the less important analytical tools. Another considerable group of scientists
have concentrated their efforts on the problems of HPLC, a method which is called
high pressure liquid chromatography but should be high performance liquid chromatography.
The separation columns - rarely operated under optimum conditions -
hardly exceed lo00 theoretical plates. Irreversible adsorption of sample components
on the column material, often limits their general application. Most of the shortcomings
of HPLC can be avoided by using TLC methods.
This book is not intended to discredit HPLC. Indeed, it is meant for those who
use HPLC. An elegant, fast, inexpensive and sufficiently precise preliminary technique
for HPLC can now be provided which may be the method of choice for
certain analytical problems. The technique has been named HPTLC, high performance
thin-layer chromatography and the editors are fully aware of the implication
of this notation. The following list of characteristics demonstrates the
potential of HPTLC.
Separation capability: up to 40 different substances, completely separated in one
single run.
separation of more than 5 components per minute (time
averaged).
- HPTLC as a preliminary technique for HPLC: analysis
of one sample or a group of substances.
- HPTLC as a routine technique: analysisof up to 12 samples
in one single run using the micro-circular technique;
analysis of up to 8 samples with the macro-circular technique
and up to 40 samples in the linear technique. Using the
continuous flow method, more than 10 samples can be
analyzed successively.
Near a Rf-value range of 0.5, real Rf-values with relative
standard deviations of f 1% can be obtained when the
circular technique (Uchamber-CAMAG) is applied.
Separation power:
Analysis capacity:
Qualitative analysis:
Quantitative analysis: Relative standard deviations of+ 2.3% or better are obtained
with the linear technique in the nanogram range. The
circular technique on 100 x 100 mm plates has relative
standard deviations of 1.5 to * 1% or better.
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